Crystal Digital PCR is Stilla’s next-generation technology for absolute quantification of nucleic acids.
Using cutting-edge microfluidic innovations, this technology integrates the digital PCR process in a single consumable. The sample is first flowed through a network of microchannels and partitioned into a large 2D array of 30,000 individual droplets, also called a droplet crystal.
PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.
With Crystal Digital PCR, the combination of powerful image analysis and intuitive visual inspection offers an unmatched level of confidence in the digital PCR measurement, yielding data you can truly trust.

Workflow

Generate crystals & Amplify, Read & Analyze

Product Features
₋Fully enclosed: Sample is joined with special chip and sealed entirely. No cross-contamination.

-Micro-flow device: The sample automatically generates and tiles 25000-30000 uniform droplets.

₋3-color channels:FAM…/VIC…/Cy5…three fluorescence channels for multiplex assay

₋crystal droplets inspection:visual inspection to the single droplet

-compatible with Evagreen, TaqMan probe and the likewise fluorescence
assayFAM, Evagreen... // Cy3, VIC, HEX, ... // Cy5, Quasar® 705, ...
NaicaTMCrystal droplets digital PCR system
The NaicaTMcrystal Digital PCR System includes dedicated consumable Sapphire Chip, Naica Geode, Naica Prism3 and Crystal Miner softwae, leveraging the key assets of Crystal Digital PCR in a compact, easy-to-use, fast and reliable digital PCR solution.